Medicine-Allergy/Rheumatology

HSC T-16-032

1) Molecular Biology and Function of C1q Receptors: cC1q-R and gC1q-R, 2) Role of gC1q-R in blood Coagulation, 3) C1q-R-mediated Phagocytosis, 4) Three Dimensional Structure of gC1q-R,(*4) Role of C1q-R in inflammation, infection, and autoimmunity.

Our laboratory is involved in the study of the function and molecular biology and three-dimensional structure of cell surface receptors or binding proteins specific for human C1q.

PCR, RT-PCR, Gene expression, Peptide mapping, Protein purification, Generation and characterization of monoclonal antibodies, Western and Northern blotting, Immunoprecipitation, Tissue culture

Moderate laboratory experience if possible, if not interest in research will do. Our lab will take care of the rest.

1.Ghebrehiwet, B., J.Jesty, and E.I.B.Peerschke. 2002. gC1q-R/p33: Structure-function predictions from the crystal structure. Immunobiol. 205:421-432. 2.Ghebrehiwet, B., X. Feng, R. Kumar, and E.I.B. Peerschke. 2003. Complement component C1q induces endothelial cell adhesion and spreading through a docking/signaling partnership of C1q receptors and integrins. International. Immunopharm. 3:299-310. 3. Rubinstein, D.B., A. Stortchevoi, M. Boosalis, R. Ashfaq, B. Ghebrehiwet, E.I.B. Peerschke, F. Calvo, and T. Gillaume. 2004. Receptor for the globular heads of C1q (gC1q-R; p33; Hyaluronan-binding protein) is preferentially expressed by adenocarcinoma cells. Internl. Cancer Res. 110:741-750. 4. Peerschke, E.I.B., J. O. Minta, S.Z.Zhou, A. Bini, A. Gotlieb, R.W.Colman and B. Ghebrehiwet. 2004. gC1q-R/p33 expression on human coronary artery atherosclerotic lesions. Molec. Immunol. 41:759-766 5. Ghebrehiwet, B., and E.I.B. Peerschke. 2004. cC1q-R (calreticulin) and gC1q-R/p33: ubiquitously expressed multi-ligand binding cellular proteins involved in inflammation and infection. Molec. Immunol 41:173-183.